A genome wide resource for RNAi in Arabidopsis. The Arabidopsis Genomic RNAi Knock-down Line Analysis project aimed to construct 4000-5000 gene specific RNAi constructs in order to help researchers to produce mutants in any gene of interest. Amplicons, entry clones and seeds generated by this project are available at NASC.
From the abstract: Gene-specific sequence tags (GSTs) were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
Database to carry out searches for insertion mutants in a number of populations including those from the John Innes Centre (ATIS), the Sainsbury Laboratory (SM) and the Cold Spring Harbour gene trap lines. Searches are carried out via a genome browser format that also provides information on homologous Brassica sequences, CATMA primer locations, micro-array probe locations and small RNA signatures.
From the abstract: We have developed a database (http://atidb.cshl.org/) for archiving, searching and analyzing insertional mutagenesis lines. Flanking sequences from approximately 10 500 insertion lines (including transposon and T‐DNA insertions) from several tagging programs in Arabidopsis were mapped to the genome sequence through our annotation system before being entered into the database.
This centre provides seeds stock for T-DNA insertion lines, a collection of natural population and recombinant inbreed lines.
A vast collection of seed stocks, including ecotypes and mutant seeds from the Sendai Arabidopsis Stock Center, RIKEN GSC Transposon-tagged line seeds, RIKEN GSC/BRC Activation (T-DNA)-tagged line seeds, RIKEN PSC Arabidopsis FOX line seeds, Individual lines (mutant and transgenic lines), and RIKEN GSC Full-length cDNA (RAFL) clones.
An overview of various forward and reverse genetics projects and seed resources.
A tool allowing the user to search for germplasms/seed stocks in the TAIR database using a number of parameters.
- Plant Expression Vectors
- Early Career Resources
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- RNA toolkit and repositories
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- General Database
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- Microarray Facilities
- Microarray Tool Kit
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- Proteomic Tool Kit
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- Deep Sequencing Tool Kit
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- Systems Biology Tool Kit