26th Nov 2013

Here’s another batch of the latest Arabidopsis papers to be made available this week, including work from the Universities of Exeter, Liverpool, Edinburgh, York, Sheffield, Nottingham and Warwick, and the John Innes Centre.


  • MacGregor DR, Gould P, Foreman J et al. HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 is required for circadian periodicity through the promotion of nucleo-cytoplasmic mRNA export in Arabidopsis. The Plant Cell, 25 October 2013. DOI: 10.1105/tpc.113.114959.

This paper involved a UK collaboration between scientists from the Universities of Exeter, Liverpool, Edinburgh and York – including GARNet committee member Anthony Hall, and former GARNet chair Andrew Millar. It has been shown that the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 (HOS1) has many more roles than previously thought. As well as degrading INDUCER OF CBF EXPRESSION1 to attenuate cold acclimation, it also appears to be involved in indirect cold signaling via disruption of the circadian clock.


  • Dorca-Fornell C, Pajor R, Lehmeier C et al. Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern. The Plant Journal, 11 November 2013. DOI: 10.1111/tpj.12342.

Scientists from Sheffield and Nottingham have identified that retinoblastoma-related protein (RBR) – a regulator of the cell cycle – is involved in mesophyll differentiation that affects tissue porosity and the distribution of air space within the leaf. RBR is therefore important in the early development of the leaf and affects how the plant’s physiology changes in response to differences in cell cycle regulation.


  • Feeney M, Frigerio L, Kohalmi SE, Cui Y and Menassa R. Reprogramming cells to study vacuolar development. Frontiers in Plant Science, 15 November 2013. DOI: 10.3389/fpls.2013.00493. [Open Access]

Mistianne Feeney and Lorenzo Frigerio from the University of Warwick (along with Canadian colleagues) present this review of the usefulness of cellular reprogramming to study processes that occur during developmental transitions. These transient stages can be difficult to study in Arabidopsis embryo or seed tissues, but reprogramming cells by genetic manipulation of more accessible tissue types can be an easier way to study changes. In particular, the review highlights the use of cellular reprogramming in the study of the transition from the lytic vesicle to the protein storage vesicle.


  • Tucker MR, Roodbarkelari F, Truernit E, Adamski NM, Hinze A, Lohmüller B, Würschum T and Laux T. Accession-specific modifiers act with ZWILLE/ARGONAUTE10 to maintain shoot meristem stem cells during embryogenesis in Arabidopsis. BMC Genomics, 20 November 2013. DOI: 10.1186/1471-2164-14-809.

This BMC Genomics paper was written by a European collaboration that also involved Nikolai Adamski from the John Innes Centre. In it, experiments to understand the impact of natural variation on shoot stem cell maintenance are described. In particular, the ZWILLE/PINHEAD/AGO10 (ZLL) gene is discussed, which in the Landsberg erecta Arabidopsis accession is essential for stem cell maintenance, whereas in Columbia its requirement is masked by genetic modifiers.